Nuclease-Assisted Minor Allele Enrichment Using Overlapping Probes-Assisted Amplification-Refractory Mutation System: An Approach for the Improvement of Amplification-Refractory Mutation System-Polymerase Chain Reaction Specificity in Liquid Biopsies
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چکیده
منابع مشابه
Multiplex Tetra-Primer Amplification Refractory Mutation System Polymerase Chain Reaction to Genotype SNP8NRG221533 of Neuregulin-1 Gene
Schizophrenia is a severe neuropsychiatric disorder with symptoms such as hallucination, delusion and mental disorder. It is a complex disorder, in which genetic components play a crucial role in its pathogenesis. Among candidate genes for schizophrenia, Neuregulin 1 (NRG1) gene is the most important gene, association of which with the illness has been confirmed in several studies. Single nucl...
متن کاملThe Quantitative Amplification Refractory Mutation System
The amplifi cation refractory mutation system (ARMS), which has also been described as allele-specifi c PCR (ASP) and PCR amplifi cation of specifi c alleles (PASA), is a PCR-based method of detecting single base mutations (Newton et al., 1989). ARMS has been applied successfully to the analysis of a wide range of polymorphisms, germline mutations and somatic mutations. The technique has the ab...
متن کاملmultiplex tetra-primer amplification refractory mutation system polymerase chain reaction to genotype snp8nrg221533 of neuregulin-1 gene
schizophrenia is a severe neuropsychiatric disorder with symptoms such as hallucination, delusion and mental disorder. it is a complex disorder, in which genetic components play a crucial role in its pathogenesis. among candidate genes for schizophrenia, neuregulin 1 (nrg1) gene is the most important gene, association of which with the illness has been confirmed in several studies. single nucl...
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AIMS To detect HLA susceptibility and protective alleles associated with insulin dependent diabetes mellitus (IDDM) using a multiplex amplification refractory mutation system (ARMS). These include DR3 and DR4 alleles at the DRB1 locus, presence or absence of aspartic acid at position 57 (Asp-57) of the DQB1 locus, and presence or absence of arginine at position 52 (Arg-52) of the DQA1 locus. ...
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Major depressive disorder (MDD) is a prevalent, debilitating mood disorder that has been associated with several genetic polymorphisms. One such polymorphism, namely that of apolipoprotein E (APOE), has three allelic forms (ε2, ε3 and ε4) that encode for six unique isoforms of the APOE protein. A growing number of techniques have been developed for APOE genotyping; however, not all polymerase c...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2019
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.9b03325